ROCK inhibition rescues the effects on the actin cytoskeleton and tight junctions of VE-cadherin down-regulation. (A) HDMEC transfected with the indicated siRNAs were analyzed by immunoblotting as labeled. Cells were transfected with control or VE-cadherin siRNAs and, 48 h later, were incubated for another 24 h in the presence or absence of the ROCK inhibitor Y27632. (B and C) Then, they were analyzed by immunofluorescence for the indicated proteins. Results shown are representative from three duplicate experiments. Bars: (B) 30 µm; (C) 40 µm.