JAM-A down-regulation redistributes ZO-1 and claudin-5, and induces focal adhesions. (A and C) Cells transfected with control or JAM-A siRNAs were analyzed by immunofluorescence for the indicated proteins. (B) Equivalent samples were analyzed by expression of the indicated proteins by immunoblotting. Results shown are representative from three duplicate experiments performed. Bars, 30 µm.