ZO-1 depletion leads to a selective redistribution of tight junction proteins and junctional mechanotransducers. (A–E) HDMEC with or without down-regulation of ZO-1 were analyzed by immunofluorescence and immunoblotting using antibodies for the indicated tight junction (A and B) and adherens junction (B and C) proteins, as well as mechanotransducers and regulators (D and E). (F–H) Control and siRNA-depleted cells were extracted and used for immunoprecipitation with antibodies against α-catenin (F), VE-cadherin (G), or talin (H). Immunoprecipitates were then analyzed by immunoblotting as indicated. Results shown are representative from three duplicate experiments. Bars: (A) 40 µm; (C) 50 µm; (D) 30 µm.