Rab13 knockdown reduces invasiveness and growth of breast cancer cells. (A) 1833TR cells with stable expression of control or Rab13 targeting shRNAs were plated on Matrigel-coated membranes for 14 h and invading cells were fixed and stained with crystal violet. Bar, 300 µm. (B) Quantification of invading cells in A. normalized to control shRNA (mean ± SEM from four independent experiments measuring 24 fields of view per condition per experiment; one-way ANOVA with Dunnett’s post-test; *, P < 0.05). (C) 1833TR cells were plated on Matrigel-coated coverslips for 14 h and stained with Hoechst, and the number of cells was quantified (mean ± SEM from four independent experiments measuring 24 fields of view per condition per experiment, one-way ANOVA). (D) 1833TR cells were trypsinized and stained with Trypan blue, and the number of viable cells was quantified (mean ± SEM from five independent experiments measuring three wells per condition per experiment; statistical analysis used as in B). (E) 1833TR cells, plated at low density were imaged every hour for 120 h. Cells detected by imaging software are highlighted in yellow. Bar, 300 µm. (F) Quantification of cell confluency over time as in E. Graph shows a representative experiment (mean ± SD from three independent experiments measuring three wells per condition per experiment; two-way ANOVA; *, P < 0.05).