Rab13 knockdown reduces migration of breast cancer cells. (A) MDA-MB-231-1833TR cells were stably transduced with lentivirus driving expression of control shRNA or three different shRNAs targeting Rab13, and the indicated proteins were detected by blotting. (B) The levels of Rab13 from experiments as in A were normalized to Hsc70 and expressed relative to control cells (mean ± SD from four independent experiments; one-way ANOVA with Dunnett’s post-test; ***, P < 0.001). (C) As in A, but using BT549 cells. (D) As in B, but quantifying knockdown in C. (E) The migration pattern of single 1833TR cells expressing control or Rab13 targeting shRNAs. The mean migration speed of all cells measured is shown in the bar graph (mean ± SEM from three independent experiments measuring a minimum of 10 cells per condition per experiment; one-way ANOVA with Dunnett’s post-test; **, P < 0.01). (F) As in E, but with BT549 cells.