Figure 6.
Figure 6. DENND2B C-terminal region is required for the morphological transformation of MCF10A cells. (A) Schematic of DENND2B constructs used in these experiments. (B) HeLa cells were transfected with the indicated GFP-DENND2B constructs, fixed, and processed for fluorescence for GFP and Phalloidin-AF647. Bar, 10 µm. (C) Protrusion length of MCF10A cells cotransfected with mCh-Rab13 and the indicated constructs was quantified (mean ± SEM from a minimum of three independent experiments measuring a minimum of 25 cells per condition per experiment; one-way ANOVA with Dunnett’s post-test; ***, P < 0.001). (D) Sequence of the 17 C-terminal residues of human DENND2B aligned to the indicated species. Identical residues are boxed. C-term, C terminus; FL, full length; N-term, N terminus.

DENND2B C-terminal region is required for the morphological transformation of MCF10A cells. (A) Schematic of DENND2B constructs used in these experiments. (B) HeLa cells were transfected with the indicated GFP-DENND2B constructs, fixed, and processed for fluorescence for GFP and Phalloidin-AF647. Bar, 10 µm. (C) Protrusion length of MCF10A cells cotransfected with mCh-Rab13 and the indicated constructs was quantified (mean ± SEM from a minimum of three independent experiments measuring a minimum of 25 cells per condition per experiment; one-way ANOVA with Dunnett’s post-test; ***, P < 0.001). (D) Sequence of the 17 C-terminal residues of human DENND2B aligned to the indicated species. Identical residues are boxed. C-term, C terminus; FL, full length; N-term, N terminus.

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