Figure 2. The DC actin cytoskeleton... | Rockefeller University Press

Figure 2.

$Figure 2. The DC actin cytoskeleton clusters ICAM-1 and constrains its mobility. (A) Mature BMDCs were treated with the indicated actin-depolymerizing agents, fixed, and labeled for cell surface MHCII and ICAM-1, followed by permeabilization and labeling for F-actin. Bar, 10 µm. (B and C) Mobile fraction (B) and diffusion coefficient (C) of MHCII and ICAM-1 on mature BMDCs treated as indicated. Dots represent individual FRAP measurements (n = 253–417) pooled from four independent experiments. *, P < 0.01; **, P < 0.001; ***, P < 0.0001.$

The DC actin cytoskeleton clusters ICAM-1 and constrains its mobility. (A) Mature BMDCs were treated with the indicated actin-depolymerizing agents, fixed, and labeled for cell surface MHCII and ICAM-1, followed by permeabilization and labeling for F-actin. Bar, 10 µm. (B and C) Mobile fraction (B) and diffusion coefficient (C) of MHCII and ICAM-1 on mature BMDCs treated as indicated. Dots represent individual FRAP measurements (n = 253–417) pooled from four independent experiments. *, P < 0.01; **, P < 0.001; ***, P < 0.0001.