Figure 1. Dendritic cells regulate the... | Rockefeller University Press

Figure 1.

$Figure 1. Dendritic cells regulate the lateral mobility of ICAM-1. (A) BMDCs were untreated (broken lines) or treated (solid lines) with 100 ng/ml LPS to induce maturation, stained for the indicated proteins, and analyzed by flow cytometry. Data are representative of three individual experiments. (B) Representative images of BMDCs labeled with Fabs against MHCII or ICAM-1 and imaged at the indicated times after photobleaching at time = 0. Bars, 1 µm. (C and D) Mobile fraction (C) and diffusion coefficient (D) of MHCII and ICAM-1 on the ventral surface of control or LPS-matured BMDCs. Dots represent individual FRAP measurements (n = 102–433) pooled from at least three independent experiments. ***, P < 0.0001.$

Dendritic cells regulate the lateral mobility of ICAM-1. (A) BMDCs were untreated (broken lines) or treated (solid lines) with 100 ng/ml LPS to induce maturation, stained for the indicated proteins, and analyzed by flow cytometry. Data are representative of three individual experiments. (B) Representative images of BMDCs labeled with Fabs against MHCII or ICAM-1 and imaged at the indicated times after photobleaching at time = 0. Bars, 1 µm. (C and D) Mobile fraction (C) and diffusion coefficient (D) of MHCII and ICAM-1 on the ventral surface of control or LPS-matured BMDCs. Dots represent individual FRAP measurements (n = 102–433) pooled from at least three independent experiments. ***, P < 0.0001.