DPP signaling is required for Jupiter, Jar, and Zasp52 LE expression during DC. (A–C‴) Embryos at stage (S) 12 (A), 13 (B), and 15 (C) displaying Jupiter::GFP (green; gray in A′, B′, and C′), Jar (red; gray in A″, B″, and C″), and Zasp52 (blue; gray in A‴, B‴, and C‴). Bars, 50 µm. (D–G) Control (D and E) and tkv8 (F and G) stage 12 embryos marked for Jupiter::GFP (green in D and F; gray in D′ and F′), Jar (red in D and F; gray in D″ and F″) and E-Cadherin (blue in D and F), or Zasp52 (green in E and G) and E-Cadherin (magenta in E and G). Bars, 10 µm. (H–H″) Plot profile of Jupiter::GFP (n = 8), Jar (n = 8), and Zasp52 (n = 10) intensity in control and tkv8 embryos. AS, amnioserosa; LE, leading edge; Lat.E, lateral epidermis. (Two-way ANOVA and Bonferroni post-hoc test: ***, P < 0.001.) Accumulation of Jupiter::GFP, Jar, and Zasp52 at the LE is lost in tkv− embryos (arrowheads). Error bars are means ± SEM.
