Figure 2.
Figure 2. Effect of α-R403A and α-E416A mutations analyzed in an MT-gliding assay. (A) Time course of the positions of single MTs in a direction parallel to the long axes of the MTs measured in the presence of 1 mM ATP. Negative displacement corresponds to motion toward the minus end of the MT. (B) Distributions of displacements at the indicated time intervals. The diffusion coefficient, calculated from the variance of the distribution, was 0.0021 and 0.0022 µm2/s for α-R403A and α-E416A, respectively (Fig. S2).

Effect of α-R403A and α-E416A mutations analyzed in an MT-gliding assay. (A) Time course of the positions of single MTs in a direction parallel to the long axes of the MTs measured in the presence of 1 mM ATP. Negative displacement corresponds to motion toward the minus end of the MT. (B) Distributions of displacements at the indicated time intervals. The diffusion coefficient, calculated from the variance of the distribution, was 0.0021 and 0.0022 µm2/s for α-R403A and α-E416A, respectively (Fig. S2).

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal