Genetic interactions between members of cER inheritance pathways. (A, top) Tetrad analyses of diploid cells heterozygous for Δepo1 and Δptc1 or Δepo1 and Δscs2. (bottom) Quantifications of the diameters of the colonies formed by single spores after 4 d (left) or 5 d (right) of growth (19 < n < 28). Error bars show SEM. ***, P < 0.001. (B) Fluorescence microscopy of Hmg1-GFP–expressing single- and double-deletion mutants derived from tetrad dissection in A. The classification of cER phenotypes into one of the five different groups is indicated below the images, and idealized drawings of their implementations are given at the bottom. Bar, 5 µm. (C) Classification of cER inheritance defects and quantification of their relative abundance in percentages of inspected WT, Δscs2, Δepo1, and Δepo1 Δscs2 cells. The values for each strain were separated according to bud length of the cells. (D) As in C, but with WT, Δptc1, Δepo1, and Δepo1 Δptc1 cells (43 < n < 220).