Epo1p is not a core component of the polarisome. (A) Comparison between the bud staining of Epo1-GFP in WT and Δpea2 cells (top) and between the Pea2-GFP staining in WT and Δepo1 cells (bottom). Insets show a reduction of the DIC image of the same cell. Bars, 3 µm. Shown on the right are the means of the intensity profiles (radial sums) of the GFP signals at the bud circumference derived from four cells each. (B, top) Quantification of FI of Epo1-GFP at the tip of small budded and the neck of large budded cells. The mean of the signal intensity at the bud tip or neck were divided by the mean cytosolic intensity measured in the mother cell. (101 < n < 108; error bars show SEM). (bottom) Relative distribution of cells showing either no, a spread, or a focused bud tip signal of Epo1-GFP. Analyzed were the same small-budded cells as in the top quantification. Shown are the means of 100 cells each. (C) Quantification of signal intensity of Pea2-GFP and Epo1-GFP relative to Spa2-GFP at the bud tip of small budded cells (42 < n < 55; error bars show SEM). (D, top) Length/width ratio of daughter cells of WT and selected single-deletion strains (200 < n < 246; error bars show SEM). (bottom) DIC pictures of representative cells of the corresponding genotypes. Bar, 5 µm. ***, P < 0.001.