Epo1p is a component of the polarisome. (A) Two-channel microscopy of yeast cells coexpressing Spa2-GFP and Kel1-Cherry (left), Epo1-GFP and Kel1-Cherry (middle), or Epo1-GFP and Spa2-Cherry (right). Each image shows polar-growing cells (left) and cells during mitosis (right). (B) Stills of a time-lapse analysis of cells coexpressing Epo1-GFP and Spa2-Cherry. Pictures start in late G2 (t = 0 min) and end with the outgrowth of a new bud (t = 48 min). (C) Stills of a time-lapse analysis of cells in late mitosis and coexpressing Spa2-GFP and Shs1-Cherry (left) or Epo1-GFP and Shs1-Cherry (middle). t = 0 min indicates the splitting of the septin rings. (right) Calculated time between the first appearance of Spa2-GFP (n = 31) or Epo1-GFP (n = 33) at the bud neck and the splitting of the septin rings (error bars show SEM). Bars: (A and B) 5 µm; (C) 1 µm.