Figure 2.
Figure 2. KIF4A is required to target PP2A–B56γ and -ε to the central spindle. (A) Thymidine-synchronized HeLa cells were transfected with GFP-tagged PP2A–B56 regulatory subunits and KIF4A siRNA for 24 h, and then, the thymidine was washed out. After 10 h, the cells were stained for DNA. PP2A–B56 was visualized by GFP fluorescence. Arrows mark PP2A–B56 associated with the central spindle. (B) Central spindle localization of GFP-tagged PP2A–B56 subunits in control and KIF4A silenced cells is plotted on a bar graph (means ± SEM, n = 3 measuring >100 cells per experiment). (C) Blots to confirm KIF4A depletion and expression of GFP-tagged PP2A–B56 subunits. Tubulin was used as a loading control. siControl, control siRNA.

KIF4A is required to target PP2A–B56γ and -ε to the central spindle. (A) Thymidine-synchronized HeLa cells were transfected with GFP-tagged PP2A–B56 regulatory subunits and KIF4A siRNA for 24 h, and then, the thymidine was washed out. After 10 h, the cells were stained for DNA. PP2A–B56 was visualized by GFP fluorescence. Arrows mark PP2A–B56 associated with the central spindle. (B) Central spindle localization of GFP-tagged PP2A–B56 subunits in control and KIF4A silenced cells is plotted on a bar graph (means ± SEM, n = 3 measuring >100 cells per experiment). (C) Blots to confirm KIF4A depletion and expression of GFP-tagged PP2A–B56 subunits. Tubulin was used as a loading control. siControl, control siRNA.

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