Normal microtubule dynamics and stability in AnkB-deficient neurons. (A) Kymographs illustrate the movement of EB1-GFP comets in AnkB^+/+ and AnkB^−/− hippocampal axons. (B and C) Quantification of the mean number (B) and mean velocity (C) of EB1-GFP comets in AnkB^+/+ (n = 25) and AnkB^−/− (n = 29) axons from five independent preparations. For all graphs, data represent means ± SEM and were obtained from one experiment out of three repeats. (D) Representative images show immunofluorescent staining of DIV10 control and AnkB^−/− neurons with antibodies to either acetylated (left), detyrosinated (center), or polyglutamylated (right) tubulin subunits.