AnkB is required for axonal growth in mouse brains and cultured hippocampal neurons. (A) FA maps of mouse brains and 3D images of white matter tracts colored by FA values. Positions of the corpus callosum (arrowheads, red boxes) and anterior commissures (asterisks, white boxes) are indicated. (B) Size distribution of axonal tracts in control and AnkB^−/− brains (n = 4 mice). The data shown represent the mean of four independent DTI scans per each genotype performed as part of one single experiment. (C) Time course of axonal growth in hippocampal neurons. Axon initial segments were labeled by ankyrin-G staining (AnkG). Axons and dendrites were labeled by neurofilament and MAP2 staining, respectively. (D and E) Quantification of axonal length at different DIVs (D) and dendritic branches length at DIV8 (E). Data were compiled from a single representative experiment out of three repeats. For the experiment shown, data represent means ± SEM; n = 40 cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001, two-tailed t test.