ROS and SFKs mediate macrophage wound attraction. (A) Representative images of 3-dpf Tg(mpeg1:Dendra2) larvae treated with increasing concentrations of the NADPH oxidase inhibitor DPI for 1, 3, and 6 hptt. (B) Quantification of macrophage recruitment to wounded fins after DPI treatment at the indicated times. (C) Representative images of control, duox morphant (duox MO), or cyba morphant (cyba MO) 3-dpf Tg(mpeg1:Dendra2) larvae fixed 1 and 6 hptt. (D) Quantification of macrophage recruitment to wounded fins of control, duox MO, or cyba MO larvae at the indicated times. (E) Representative images of 3-dpf Tg(mpeg1:Dendra2) larvae treated with 20 µM SFK inhibitor PP2 for 1 and 6 hptt. (F) Quantification of macrophage recruitment to wounded fins obtained in E. (G) Representative images of wild-type (control) 3-dpf Tg(mpeg1:Dendra2) larvae and yrk morphants #1 and #2 (yrk MO1 and yrk MO2) larvae fixed 1 and 6 hptt. (H) Quantification of macrophage recruitment to wounded fins in control, yrk MO1, and yrk MO2 larvae at 1 and 6 hptt. Ctrl, control. (n) = number of larvae. Horizontal lines indicate means. Error bars indicate SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, one-way ANOVA with Dunnett’s post-test (B and H) and two-tailed unpaired t test (D and F). Dashed lines border the location of tail transection. Bars: (A and C) 60 µm; (E and G) 50 µm.