Figure 1.

Kinetics of neutrophil–macrophage interactions at wounds. (A) Representative images of 3-dpf double transgenic larvae Tg(mpx:DsRed) × Tg(mpeg1:Dendra2) showing neutrophil and macrophage recruitment and resolution at the indicated times after needle wounding. (B) Quantification of the number of neutrophils and macrophages at wounds of larvae depicted in A. n = 43 larvae. Error bars indicate SEM. (C) Number of macrophages as a function of number of neutrophils at 1 and 2 hpw depicted in A. n = 43 larvae. Gray, macrophages and neutrophils are equally present at wound; green, macrophages predominate; pink, neutrophils predominate. (D) Real-time imaging of the leukocyte response to needle wounding using 3-dpf double transgenic Tg(mpx:DsRed) × Tg(mpeg1:Dendra2) larvae. Dotted lines delimit fins. Diamonds indicate neutrophil #1, and asterisks indicate neutrophil #2. Triangles indicate needle wound location. Bars, 50 µm.

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