Expression of chiA gene is not concurrent with a lysis event. Representative still frames (separate DIC and FITC channels) taken from time-lapse videos for strain FTG005 (ϕchiA::gfp) demonstrating the two fates of GFP-positive cells upon time-lapse imaging. The S. marcescens FTG005 (ϕchiA::gfp) strain was grown for 18 h in minimal media before being diluted and applied to a microscope slide. (A) FITC-positive cells divide and propagate. The asterisks highlight a single cell growing and dividing, twice, into daughter cells. (B) A subpopulation of FITC-positive cells remains fluorescent but does not divide even over an extended period of time. Progression from left to right is with respect to time, as noted on the micrograph (minutes). The left-hand asterisks highlight a quiescent cell that does not divide. The right-hand asterisks highlight a single cell growing and dividing, twice, into daughter cells. Bars, 10 µm. Videos of these images (merged) are available as Videos 1 and 2.