Figure 1.
![Figure 1. S. marcescens chitinases are extracellular proteins. S. marcescens Db10 was grown aerobically in rich medium. At the time points indicated, an aliquot was taken and separated into culture supernatant (SN) and whole cells (WC) by centrifugation. Samples were analyzed for the presence of ChiA, ChiB, and ChiC by Western immunoblotting. The locations of a periplasmic control protein (maltose binding protein [MBP]) and a cytoplasmic control protein (RNA polymerase [RNAP]) are shown.](https://cdn.rupress.org/rup/content_public/journal/jcb/207/5/10.1083_jcb.201404127/6/jcb_201404127_fig1.jpeg?Expires=1771766403&Signature=AezXsQgNrdpqeHufWaKocDmMUQKMw92DtS~VMG95L3JmRD7H670b2n0lM6Rmt~SIGbmrWpxW0GVan-vvNV2qukmhOs24TNFgsVrrycOgBeE8TlcYz4gQYNyIu-eC62Mxez34UAhheHu0Dm7DtR0x6dP~SKycVbS3dyeF9za8DxclpQKWe8yCGGPuccQ5vt77rD7PVNaNPWM3dINYEp3e1J-ilGqLbuhUt9BkI~APy7Z0BYTjCRKL3qm1PCgTpp-xNInGkWTUv013epoBJQs~x4LTDXczYkVYLVA8uVLqCSvqdi7wbFOJd1KNz1iJ-kj1sG8eMC1HfYwktAuwcLbqdA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
S. marcescens chitinases are extracellular proteins. S. marcescens Db10 was grown aerobically in rich medium. At the time points indicated, an aliquot was taken and separated into culture supernatant (SN) and whole cells (WC) by centrifugation. Samples were analyzed for the presence of ChiA, ChiB, and ChiC by Western immunoblotting. The locations of a periplasmic control protein (maltose binding protein [MBP]) and a cytoplasmic control protein (RNA polymerase [RNAP]) are shown.
