Figure 4.
Figure 4. Reversible IFNα2 binding to both IFNAR1 and IFNAR2. (a) HaloTag-IFNAR2 expressed in HeLa cells together with IFNAR1-EGFP (green channel) and cultured on a micropatterned support at different times after injection of AT655IFNα2 (red channel). Bars, 10 µm. (b) Micropatterns after binding of 0.5 nM DY647IFNα2 M148A (left) and after addition of unlabeled IFNα2-α8tail-YNS (right). Dotted lines indicate the analyzed line pattern. Bars, 5 µm. (c) Displacement kinetics in micropatterns after addition of unlabeled IFNα2-α8tail-YNS and a monoexponential fit of the curve (from the cell shown in b, representative of three cells analyzed). The time regimen during injection and refocusing is marked by a gray bar.

Reversible IFNα2 binding to both IFNAR1 and IFNAR2. (a) HaloTag-IFNAR2 expressed in HeLa cells together with IFNAR1-EGFP (green channel) and cultured on a micropatterned support at different times after injection of AT655IFNα2 (red channel). Bars, 10 µm. (b) Micropatterns after binding of 0.5 nM DY647IFNα2 M148A (left) and after addition of unlabeled IFNα2-α8tail-YNS (right). Dotted lines indicate the analyzed line pattern. Bars, 5 µm. (c) Displacement kinetics in micropatterns after addition of unlabeled IFNα2-α8tail-YNS and a monoexponential fit of the curve (from the cell shown in b, representative of three cells analyzed). The time regimen during injection and refocusing is marked by a gray bar.

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