Figure 3.
Figure 3. Ternary complex assembly in micropatterns. (a and b) HaloTag-EGFP-IFNAR1 (green channel) expressed in HeLa cells together with TagRFP-IFNAR2 (yellow channel) and cultured on a micropatterned support before (a) and 20 min after (b) addition of AT655IFNα2 (red channel). Bars, 10 µm. (c) Distribution IFNAR1 (green), IFNAR2 (orange), and IFNα2 (red) after formation of the ternary complex (representative of two cells analyzed). For comparison, the distribution of IFNAR2 before addition of IFNα2 (orange, dotted line) is shown. Integrated line profiles were scaled to similar amplitudes and overlaid at an arbitrary ordinate scale. a.u., arbitrary unit. (d) Time-resolved increase of the IFNAR2 concentration within (black squares) and decrease outside IFNAR1 micropatterns (red dots). Intensities normalized to the intensities outside the functionalized areas are plotted (representative of two cells analyzed).

Ternary complex assembly in micropatterns. (a and b) HaloTag-EGFP-IFNAR1 (green channel) expressed in HeLa cells together with TagRFP-IFNAR2 (yellow channel) and cultured on a micropatterned support before (a) and 20 min after (b) addition of AT655IFNα2 (red channel). Bars, 10 µm. (c) Distribution IFNAR1 (green), IFNAR2 (orange), and IFNα2 (red) after formation of the ternary complex (representative of two cells analyzed). For comparison, the distribution of IFNAR2 before addition of IFNα2 (orange, dotted line) is shown. Integrated line profiles were scaled to similar amplitudes and overlaid at an arbitrary ordinate scale. a.u., arbitrary unit. (d) Time-resolved increase of the IFNAR2 concentration within (black squares) and decrease outside IFNAR1 micropatterns (red dots). Intensities normalized to the intensities outside the functionalized areas are plotted (representative of two cells analyzed).

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