Figure 2.
Figure 2. Spectra and pH titration curves of pH-sensitive FPs. (A) Excitation and emission spectra of pH-sensitive FPs SEP, pHTomato, pHoran4, and pHuji at pH 5.5 (dashed line) and 7.5 (solid line). (B) pH titration curves of the indicated FP. (C) Fluorescence of HeLa cells expressing pDisplay proteins fused to the indicated FP as a function of pH. Error bars represent SEM.

Spectra and pH titration curves of pH-sensitive FPs. (A) Excitation and emission spectra of pH-sensitive FPs SEP, pHTomato, pHoran4, and pHuji at pH 5.5 (dashed line) and 7.5 (solid line). (B) pH titration curves of the indicated FP. (C) Fluorescence of HeLa cells expressing pDisplay proteins fused to the indicated FP as a function of pH. Error bars represent SEM.

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