Appendages are not detectable in early mitosis of HeLa cells analyzed by EM. (A and B) Quantification of centrosome-associated Cep164 and Odf2. The error bars represent the means and SD. NEB, nuclear envelope breakdown. (C) Four serial sections of two diplosomes in prophase. Two MCs are sectioned longitudinally, and appendages are not detectable. DCs are in an oblique section. (D) Four serial sections of centrosomes in early telophase. Weak densities (arrowheads) are visible toward the distal parts of the MCs indicative of appendage reformation. meta, metaphase; ana-telo, anaphase-telophase.