Figure 1.
Figure 1. Expression of Plk1TD in Emi1-d U2OS cells induces disengagement of short procentrioles that gradually biochemically mature. (A) EM analysis of an Emi1-d Plk1TD-expressing U2OS cell with newly disengaged DCs. Four C1-GFP signals indicate the presence of four centrioles. EM analysis of the same centrioles reveals two longitudinally sectioned DCs (∼260 nm DC1 and ∼220 nm DC2) in the vicinity of two MCs in cross section. (B and C) Localization of Centrobin, Cep152, Cep164, and Odf2 on the centrioles of Emi1-d cells, with or without Plk1TD expression. (D) Quantification of the data presented in B and C. n = 300, from three independent experiments. (E) Multiple microtubule nucleation sites, associated with C1-GFP (yellow arrows), form after nocodazole washout, which indicates that disengaged short centrioles organize functional PCM. Error bars represent the means and SD.

Expression of Plk1TD in Emi1-d U2OS cells induces disengagement of short procentrioles that gradually biochemically mature. (A) EM analysis of an Emi1-d Plk1TD-expressing U2OS cell with newly disengaged DCs. Four C1-GFP signals indicate the presence of four centrioles. EM analysis of the same centrioles reveals two longitudinally sectioned DCs (∼260 nm DC1 and ∼220 nm DC2) in the vicinity of two MCs in cross section. (B and C) Localization of Centrobin, Cep152, Cep164, and Odf2 on the centrioles of Emi1-d cells, with or without Plk1TD expression. (D) Quantification of the data presented in B and C. n = 300, from three independent experiments. (E) Multiple microtubule nucleation sites, associated with C1-GFP (yellow arrows), form after nocodazole washout, which indicates that disengaged short centrioles organize functional PCM. Error bars represent the means and SD.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal