Absence of Ensa protein expression in OoMastl^−/− oocytes. (A) Expression of Mastl, Ensa, and Arpp19 in ovulated OoMastl^+/+ and OoMastl^−/− oocytes. Lysate from 100 oocytes was loaded in each lane. (B) Relative mRNA levels of Ensa and Arpp19 in ovulated oocytes were determined by real-time PCR. The mRNA levels in the OoMastl^+/+ oocytes were set as 1.0, and the fold changes in OoMastl^−/− oocytes are shown. Error bars represent SDs.