Figure 2.
Figure 2. Failure of OoMastl−/− oocytes to enter MII after PBE. (A) OoMastl+/+ oocytes formed MetII spindles with condensed chromosomes (C) after PBE. (B) OoMastl−/− oocytes extruded PB1s but formed nuclei with decondensed chromatin. In some oocytes (left inset), the nuclei had already formed, whereas the residual central spindle microtubules remained between the chromatin in the PB1 and the nucleus. 50 oocytes from each group were analyzed, and representative images are shown. (C) The percentages of oocytes with both a PB1 and a nucleus. For A–C, ovulated (16 h after hCG) oocytes were used. (D) Representative still images from Videos 1 and 2. Timestamps indicate hours and minutes after release from IBMX. H2B-mCherry (red fluorescence) shows the DNA, and Map7-EGFP (green fluorescence) labels the spindle microtubules. Arrows indicate the positions of PB1s.

Failure of OoMastl−/− oocytes to enter MII after PBE. (A) OoMastl+/+ oocytes formed MetII spindles with condensed chromosomes (C) after PBE. (B) OoMastl−/− oocytes extruded PB1s but formed nuclei with decondensed chromatin. In some oocytes (left inset), the nuclei had already formed, whereas the residual central spindle microtubules remained between the chromatin in the PB1 and the nucleus. 50 oocytes from each group were analyzed, and representative images are shown. (C) The percentages of oocytes with both a PB1 and a nucleus. For A–C, ovulated (16 h after hCG) oocytes were used. (D) Representative still images from Videos 1 and 2. Timestamps indicate hours and minutes after release from IBMX. H2B-mCherry (red fluorescence) shows the DNA, and Map7-EGFP (green fluorescence) labels the spindle microtubules. Arrows indicate the positions of PB1s.

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