DP promotes membrane localization of Cx43. (A) Cx43 membrane localization (arrows) in NHEKs electroporated with Ct, DP, or EB1 siRNA. Thresholded Cx43 images are shown to highlight Cx43 membrane localization. Western blot is shown below. (B) Triton X-100 solubility of Cx43 in Ct or DP KD NHEKs, quantified in a graph (n = 3 independent experiments). Lines indicate that intervening lanes have been spliced out. (C) Cx43 membrane localization in Ct or DP KD organotypic raft cultures. Hematoxylin and eosin (H&E) panels demonstrate overall tissue architecture (lines mark dermal-epidermal junctions). Graph: quantification of Cx43 membrane localization (n = 15 image fields from rafts prepared in three independent experiments, with >150 regions of cell–cell contact calculated per condition). A Western blot is shown. (D) Cx43 membrane localization in Ct or DP KD primary NRVCMs. Graph: localization of Cx43 at membranes, marked by Pg (n > 95 regions of cell–cell contact from three independent experiments). Western blot shows protein expression levels. Bars, 10 µm. Error bars indicate SEM.