DP regulates MT organization and dynamics. (A) Immunostaining of MTs, DP, Pg, and nuclei (DAPI) in Ct, DP, or Dsg2 KD SCC9s. (B) Immunostaining of DP and MTs, with corresponding thresholded MT images, for Ct or DP KD SCC9s treated with 1 µM demecolcine. Lines mark approximate regions of cell–cell contact. (B′) Tubulin intensities during demecolcine treatment (n = 9 image fields). (C) MT polymerization rates and frequency of MT pausing in cytoplasmic and cortical regions of Ct or DP KD SCC9s (n > 119 EB3 tracks for each condition compiled from more than seven cytoplasmic or cortical fields from more than four movies for each condition). All data are from SCC9s. Bars, 10 µm. Error bars indicate SEM.