EB1 is a novel binding partner of the DP N terminus. (A) Schematic of DP outlining residues comprising DP-NTP, the DP N-terminal hotspot for pathogenic mutations, and DP N-terminal truncation constructs (see Fig. S1 A). (B) Yeast two-hybrid using DP-NTP as bait. Myr and MyrSB were used as negative and positive controls, respectively, and the known DP-NTP binding partner Pg was used as a positive control. Colony growth on Gal but not Glu plates at 37°C indicates a positive interaction. (C) Pull-down analysis of FLAG-tagged DP-NTP expressed in HEKs with recombinant His-tagged full-length EB1 (EB1-FL), His-tagged EB1 N terminus (residues 1–184), or uncoupled nickel (Ni-NTA) beads. (D) Immunoprecipitation of DP and coimmunoprecipitation of EB1 from NHEKs. Nonspecific rabbit IgG (Rb IgG) was used as a negative control.