ALiX and Hrs have different effects on SC growth. (A) Growth of SCs relative to MCs in 6-d-old males expressing different transgenes in SCs, as measured by the ratio of SC to MC nuclear size and normalized to controls. Values inside bars show number of males tested (n). DN, dominant negative. (B–D) SCs in 6-d-old males expressing either GFP linked to a nuclear localization sequence (GFP^NLS) alone (B) or in combination with either Hrs-RNAi (C) or ALiX-RNAi (D), under the control of the esgF/O^ts driver. Control SC nuclei are smaller than MC nuclei at eclosion but grow to be larger by 6 d (B). Data for A were analyzed using the Student’s t test or Mann–Whitney U test (*, P < 0.05; **, P < 0.01; ***, P < 0.001, n = 10) after a Shapiro–Wilk test for normality. Error bars indicate ±SE. Bars, 10 µm.