Figure 1.
Figure 1. Ligand affinity of sperm GC. (A) Dose–response relation of resact binding. The continuous line represents a fit of the Hill equation to the data from a total of 126 experiments. Each data point represents the mean ± SD of at least three experiments. The constant of half-maximal binding was K1/2 = 0.65 ± 0.08 nM, and the Hill coefficient was n = 0.49 ± 0.03. The occupancy at ∼750 nM resact was set to 95% (31 experiments; red dot). (B) Dose–response relation at low resact concentrations. The solid line was calculated using a simple binding model using a Kd of 250 pM.

Ligand affinity of sperm GC. (A) Dose–response relation of resact binding. The continuous line represents a fit of the Hill equation to the data from a total of 126 experiments. Each data point represents the mean ± SD of at least three experiments. The constant of half-maximal binding was K1/2 = 0.65 ± 0.08 nM, and the Hill coefficient was n = 0.49 ± 0.03. The occupancy at ∼750 nM resact was set to 95% (31 experiments; red dot). (B) Dose–response relation at low resact concentrations. The solid line was calculated using a simple binding model using a Kd of 250 pM.

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