Cell form transition occurs during cell division, and the resulting epimastigote-like daughters have repositioned BB and associated organelles. (A) SEM of a dividing trypomastigote. Both flagella are attached almost along their entire length to the cell body and tip of the new flagellum is linked to the old flagellum by the FC (arrow). (B) SEM of a dividing ClpGM6 RNAi-induced cell, in which long sections of both old and new flagella are free but connected by the FC (arrow). (C) SEM of a dividing 24 h ClpGM6 RNAi-induced cell. The distal tip of the free new flagellum is linked to the attached old flagellum by the FC (arrow). (D–F) Images of a late mitotic 2K2N cell from a 24-h ClpGM6 RNAi-induced population showing that transition to an epimastigote-like morphology happens in the portion of the cell where the new flagellum and FAZ are being built. Arrows indicate the proximal end of the new FAZ. (D) IF signal of the anti-ClpGM6 antibody. (E) Phase image. (F) Phase image overlaid with IF signal of anti-ClpGM6 (red) and DAPI fluorescence (magenta). (G) TEM image of a FP area in an uninduced cell. K, kinetoplast; FP, flagellar pocket; G, Golgi; N, nucleus. (H) TEM image of an FP area in a ClpGM6 RNAi-induced cell. Labels as in G. Note the changed location of the kinetoplast, the FP, and Golgi in respect to the nucleus. Bars: (A, also for B and C) 2 µm; (D, also for E and F) 4 µm; (G and H) 1 µm.