Stabilized apical MTs are required for radial intercalation of MCCs and ICs. (A and B) Anti-acetylated tubulin antibody staining of wild-type MCCs (A, arrowheads) and of a mosaic tissue showing a control and a DN-Par3-GFP–expressing MCC (B, arrowheads) during intercalation. Maximum-intensity projection (top) and corresponding cross section (bottom) are shown. (C and D) Images (C) of MCCs stained with an antibody marking acetylated α-tubulin in DMSO (left, arrowhead), which is lost following treatment with 1 μM Nocodazole (right, arrowhead), along with quantification of intercalation (D). (E and F) Images of IC marker AE1 in DMSO (left)- and Nocodazole (right)-treated ICs (E), along with quantification of intercalation (F). Quantifications of the Nocodazole phenotypes in D and F are statistically significantly different from controls (P < 0.0001, see Table S1). Bars, 5 µm.