Apical positioning of centrioles and intercalation requires the Par complex. (A) Schematic representation of MCC intercalation. (B and C) Apical localization (arrowheads) of Par3-GFP (B) and aPKC-GFP (C) at different stages of intercalation (early and late). (D) Schematic of the quantification of centriole positioning in MCCs. (E and F) Representative z stack cross-section images (E) and quantification (F) of centriole position in control, DN-Par3, and aPKC-KD MCCs. (G) Mosaic image showing wild-type MCC that has intercalated (arrowhead) and a DN-PAR3-GFP (arrow) MCC that failed to intercalate. (H) Images illustrating the three different phenotypes used for scoring MCC intercalation. (I) Quantification of intercalation in MCCs expressing GFP, Par3-GFP, aPKC-GFP, Par-3 MO, DN-Par3-GFP, or aPKC-KD-GFP. For all experiments, cells from a total of at least five embryos from at least two independent experiments were quantified unless specified otherwise. Quantification in F is based on at least 10 cells each from a total of at least five embryos from at least two independent experiments. Quantifications of DN-Par3 and aPKC-KD phenotypes in F and I are statically significantly different from controls (P < 0.0001, see Table S1). Side projection refers to side views of projection along the x axis in all figures. See Fig. S1. Bars, 5 µm.