Inhibition of N-cadherin restores migration of LPAR2 morphant cells through interstices in vitro and in vivo. (A–D) Invasion assay with LPAR2MO cells (A and C) or LPAR2MO cells incubated with N-cadherin blocking antibody (NCD2; B and D). (E) Quantification of the invasion assay shown in A–D. 50 µm, n = 27 explants from four independent experiments. One-way ANOVA: P < 0.0001. 150 µm, n = 36 explants from four independent experiments. One-way ANOVA: P < 0.0001. Individual comparisons: ***, P < 0.001. Error bars indicate SD. (F) Grafting procedure. (G–J) Grafts of NC injected with FDx (G), FDx and LPAR2MO directly into control hosts (H), FDx and LPAR2MO treated with N-cadherin antibody (NCD2) before transplantation (I), or LPAR2MO cells overexpressing CA-RAb5 (J). Asterisks indicate eyes. (K) Analysis of NC migration of the experiment shown in G–J. 35 embryos were analyzed. One-way ANOVA: P < 0.0001. Individual comparisons: **, P < 0.01; ns, not significant. Bars: (A–D) 50 µm; (G–J) 0.5 mm.