Inhibition of cell–cell adhesion rescues LPAR2 loss of function. (A–F) Cell dispersion assay in controls (A), LPAR2MO cells (B), or LPAR2MO cells in low calcium/magnesium conditions (C), coinjected with N-Cadherin MO (D), incubated with N-Cadherin blocking antibody (E), or coinjected with activated Rab5 (F). (G) Dispersion analysis using Delaunay triangulation from experiments shown in A. n = 103 explants from 12 independent experiments. One-way ANOVA: P < 0.0001. Individual comparison: ***, P < 0.001; ns, not significant. (H) Spatial correlation analysis. The broken line represents the solid-to-fluid transition. (I) Quantification of the spatial correlation analysis; the plot shows distances at which the alignment of velocity vectors falls below 0.5 for each experimental condition. n = 17 explants from three independent experiments. One-way ANOVA: P < 0.0001. Individual comparisons: ***, P < 0.01.