Lack of LPAR2 signaling generates increased tension at cell–cell junctions. (A–C) Vinculin immunostaining in Xenopus NC cells. Vinculin localizes at cell–cell junctions (arrowheads in B) and at focal adhesions (arrowheads in C). (D) Immunostaining against Phospho-Paxillin. Nuclei are stained with DAPI (blue). Bars: (A) 50 µm; (B–D) 15 µm. (E) Diagram depicting how the VinTS FRET probe works. (F) Vinculin FRET at the cell–cell contacts with control FRET probe (Mod, blue) and VinTS in controls (green) or low cell–cell adhesion conditions (brown). n = 76 cells from three independent experiments. One-way ANOVA: P < 0.001. Individual comparison: *, P < 0.05; **, P < 0.01. (G) Vinculin FRET at the cell–cell contacts in controls (green) and LPAR2MO cells (magenta). n = 64 cells from three independent experiments. Student’s two-tailed t test: P < 0.001. (H) Vinculin FRET in focal adhesions in controls (green) and LPAR2MO cells (magenta). n = 22 cells from three independent experiments. Student’s two-tailed t test: P = 0.53.