LPAR2 signaling is required for solid-like–to–fluid-like transition during NC EMT. (A–D) Invasion assay with microfluidic tunnels. Bars, 50 µm. (E) Quantification of invasion assay (19 10-µm explants from seven independent experiments; Student’s two-tailed t test: P = 0.279; 31 25-µm explants from nine independent experiments; Student’s two-tailed t test: P = 0.006; 22 50-µm explants from seven independent experiments; Student’s two-tailed t test: P = 0.0018; 29 150-µm explants from eight independent experiments; Student’s two-tailed t test: P < 0.001). (F–I) Spatial correlation kymographs. Projection of all time points (F) or temporal maps (H) where each line corresponds to a mean of three consecutive time points. Hot colors indicate a high level of correlation between two compared velocity vectors. (G) Comparison of correlation kymographs projections between control (left) and LPAR2MO (right). (I) Comparison of correlation kymographs over time between control (top) and LPAR2MO (bottom) cells. The broken line in the control data indicates at which distance between cells correlation is lost. That represents the threshold of solid-like–to–liquid-like behavior transition.