Figure 3.
Inhibition of LPAR2 does not affect cell motility or chemotaxis. (A and B) Cell tracking analysis on cell clusters (A; n = 2,261 cells from four independent experiments; Student’s two-tailed t test: P = 0.25) and single cells (B; n = 1,299 cells from three independent experiments; Student’s two-tailed t test: P = 0.75). (C and D) Chemotaxis assay with controls (C) and LPAR2MO cells (D). Time projection of cell tracks color-coded according to their Chemotaxis index (from blue [0.1] to red [0.9]). (E) Chemotaxis index from experiments shown in C and D (mean chemotactic index of 22 explants from three independent experiments; Student’s two-tailed t test: P = 0.1837).