Figure 2.
Figure 2. A cdc42 mutant is defective in the axial budding pattern. (A) Budding pattern (percentage) of each haploid strain and cdc42-101 carrying each plasmid at 30°C. Budding pattern was determined as in Fig. 1 A. (B) 10-fold serial dilutions of WT and cdc42 mutants carrying each plasmid were compared for growth at 24 or 37°C (SC-Ura for 4 d). (C) The WT and cdc42-101 strains expressing AXL1-TAP were subjected to TAP purification. Lysates (left) and purified proteins (right) were analyzed by immunoblotting with anti-Bud4 antibodies.

A cdc42 mutant is defective in the axial budding pattern. (A) Budding pattern (percentage) of each haploid strain and cdc42-101 carrying each plasmid at 30°C. Budding pattern was determined as in Fig. 1 A. (B) 10-fold serial dilutions of WT and cdc42 mutants carrying each plasmid were compared for growth at 24 or 37°C (SC-Ura for 4 d). (C) The WT and cdc42-101 strains expressing AXL1-TAP were subjected to TAP purification. Lysates (left) and purified proteins (right) were analyzed by immunoblotting with anti-Bud4 antibodies.

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