ANI-2 is found at the rachis bridge of all germ cells throughout larval development. (A) Mid-section confocal images of the germline of wild-type hermaphrodites expressing GFP::ANI-2 (green, yellow arrowheads) and a membrane marker (red) at various stages of development. For simplicity, only one gonad arm is shown from the L3 stage onward. In all frames, anterior is to the left. Bar, 10 µm. The regions delineated by the white dashed squares are magnified in the inset (bar for insets, 5 µm). (B) Schematic representation of germline development at the developmental stages shown in E. Germ cells undergo proliferation and differentiation during larval growth until animals reach adulthood. For simplicity, only the germline is depicted.