ANI-2 stably accumulates at the midbody between the two primordial germ cells. (A) Schematic representation of germ cell specification (black nuclei) during embryonic development. The primordial germ cells Z2 and Z3 are born from the P4 blastomere and do not undergo further division until hatching. (B) Mid-section confocal images of fixed wild-type embryos immunostained with ANI-2 (green), P-granules (PGL-1, gray), PAR-4 (to label the cortex, red), and DAPI (blue). Arrows point to the germline blastomeres. Bar, 10 µm. (C) Confocal projections of the dividing P4 blastomere in fixed wild-type embryos immunostained with ANI-2 (green), P-granules (PGL-1, red), and DAPI (blue). The white dashed line delineates the cell membrane. Bar, 5 µm. (D) Mid-section confocal images of the primordial germ cells of a fixed wild-type embryo immunostained with ANI-2 (green), α-tubulin (red), and DAPI (blue). Arrows point to the midbody. Bar, 5 µm. (E) Mid-section confocal images of fixed wild-type (top), pie-1(RNAi) (middle), and mep-1(RNAi) (bottom) embryos immunostained with ANI-2 (green), α-tubulin (red), and DAPI (blue). In all frames, anterior is to the left. Bar, 10 µm.
