Figure 5.
Free actin barbed end formation is impaired in talin- and moesin-depleted cells. (A and B) Barbed end formation assay. (A) Representative images of control and talin-depleted cells stained for barbed ends (anti-biotin), cortactin, and Arp2. Invadopodium precursors are defined as cortactin-/Arp2-rich punctate structures. Blue arrowheads denote invadopodium precursors. Inset shows magnified image of invadopodia in the box. Bars: (main panel) 10 µm; (inset) 1 µm. (B) Quantification of barbed end intensity within invadopodium precursors after stimulation with 2.5 nM EGF for 0 (unstimulated) or 3 min. n > 80 invadopodia; three independent experiments. *, P = 0.0247; ***, P < 4.52 × 10−4. Error bars represent the SEM.
