Figure 9.
Localization of STB plasmid foci with respect to Rap1 in the ndj1Δ strain. (A) Chromosome spreads prepared from pachytene stage cells of the ndj1Δ strain (at 7.5 h into meiosis) were assayed for the fraction of STB plasmid foci colocalized with Rap1 foci (B) and that associated with chromosomes (C). Within the latter class, plasmid foci located at chromosome tips were demarcated (D). These data were collected by screening 22 spreads comprising 180 plasmid foci. The histograms for the wild-type strain in B–D were taken from Fig. 3 H (5.5 h), Fig. 2 D, and Fig. 2 E, respectively. The error bars indicate ± SEM. *, P < 0.05 (two-tailed t test). Bars, 2 µm.