Characterization of STB plasmid movements during prophase. In this through-focus time-lapse analysis of wild-type cells (Conrad et al., 2008), at least 35 plasmid foci (tagged by green fluorescence) were traced over a period of 2 min at 1 frame/2 s. The relative positions of four individual plasmid foci (marked 1–4) in a single nucleus at 10-s intervals are displayed at the top. Bars, 2 µm. Chromosome VIIL TELs tagged by red fluorescence were also traced over the 2-min period (1 frame/2 s) as an internal standard (shown in Fig. S3). The speeds and bias of the STB plasmid were plotted alongside those for a CEN-based plasmid harboring the TEL repeat (CEN-ARS-TEL; A–C), the chromosome IVR TELs in the unpaired (uTEL; A–C) and paired (pTEL; D–F) states, and for a CEN-based ARS plasmid (CEN-ARS; G–I). The values for chromosome dynamics used in these plots and those in Fig. 6 and Fig. S3 were taken from previously published results (Conrad et al., 2008).