Figure 4. Localization of Rep1 with... | Rockefeller University Press

Figure 4.

$Figure 4. Localization of Rep1 with respect to the STB reporter plasmid and Zip1. (A) In chromosome spreads prepared from pachytene cells (5.5 h in sporulation medium), Rep1 was visualized along with the STB reporter plasmid. (B) Rep1 foci were detected using an antibody to the native protein, with Zip1 as the chromosome marker. Bars, 2 µm. (C) The histogram at the left represents the fraction of Rep1 foci (from a total of >125 analyzed) that were localized on chromosomes (<0.4 µm separation). Within this subset, the fraction present at chromosome tips (the boxed regions, shown in 3× enlarged views on the right, depict examples of such Rep1 foci) was plotted in the histograms at the right. The error bars indicate ± SEM.$

Localization of Rep1 with respect to the STB reporter plasmid and Zip1. (A) In chromosome spreads prepared from pachytene cells (5.5 h in sporulation medium), Rep1 was visualized along with the STB reporter plasmid. (B) Rep1 foci were detected using an antibody to the native protein, with Zip1 as the chromosome marker. Bars, 2 µm. (C) The histogram at the left represents the fraction of Rep1 foci (from a total of >125 analyzed) that were localized on chromosomes (<0.4 µm separation). Within this subset, the fraction present at chromosome tips (the boxed regions, shown in 3× enlarged views on the right, depict examples of such Rep1 foci) was plotted in the histograms at the right. The error bars indicate ± SEM.