Localization of the STB reporter plasmid with respect to CEN- and TEL-specific marker proteins. Chromosome spreads were prepared at 3.5 h (leptotene/zygotene) and 5.5 h (pachytene) into meiosis. Plasmid foci were mapped with Ctf19 (A, C, and D) as the CEN marker and Mps3 (B, E, and F) or Rap1 (G and H) as the TEL marker. The antibodies for visualizing Ctf19 (CTF19-MYC), Mps3 (MPS3-HA), and Rap1 (RAP1-RFP) were anti-Myc, anti-HA, and anti-RFP, respectively. In each localization assay, ∼150 plasmid foci were scored. Bars, 2 µm.