Figure 8.
Figure 8. In the absence of p14–MP1 endosomal signaling, down-regulation of IQGAP1 by RNAi rescues FAs and cell migration. (A) IF: anti-Paxillin and anti-IQGAP1 antibodies. (B) Left: quantification of average FA length in MEFs transfected with control or IQGAP1 RNAi. Mean in percent ± SEM is compared with control p14f/− MEFs transfected with control RNAi (taken as 100%). Right: calculation of speed of migration in wound-healing assays. Mean in µm/h ± SEM. Importantly, only cells that had strong down-regulation of IQGAP1 (no signal on IF using anti-IQGAP1 antibodies as in A) were used for the calculation. See also Table S1.

In the absence of p14–MP1 endosomal signaling, down-regulation of IQGAP1 by RNAi rescues FAs and cell migration. (A) IF: anti-Paxillin and anti-IQGAP1 antibodies. (B) Left: quantification of average FA length in MEFs transfected with control or IQGAP1 RNAi. Mean in percent ± SEM is compared with control p14f/− MEFs transfected with control RNAi (taken as 100%). Right: calculation of speed of migration in wound-healing assays. Mean in µm/h ± SEM. Importantly, only cells that had strong down-regulation of IQGAP1 (no signal on IF using anti-IQGAP1 antibodies as in A) were used for the calculation. See also Table S1.

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