IQGAP1 and MP1 interaction. (A) Reciprocal co-immunoprecipitation of myc6-MP1 and IQGAP1. Cell extracts were prepared from myc6-MP1–expressing p14f/− MEFs. IP: with anti-myc (lane 2) and anti-IQGAP1 (lane 3) polyclonal antibodies; IgG control (lane 1). WB: anti-IQGAP1 (top) and anti-myc (bottom) antibodies, respectively. Black arrow indicates myc6-MP1 signal. (B) Immunoprecipitation of endogenous MP1 and p18. Cell extracts were prepared from HeLa cells. IP: with anti-IQGAP1 (lane 3) polyclonal antibody; cell lysates (lane 1), IgG control (lane 2). WB: anti-IQGAP1 (top), anti-p18 (middle), and anti-MP1 (bottom) antibodies, respectively. (C) Top: HeLa protein lysates were incubated with GST-MP1 or GST alone immobilized on glutathione-Sepharose beads. WB: anti-IQGAP1 antibody. Lysates not subjected to GST pull-down were processed in parallel (HeLa lysate). Bottom: HeLa protein lysates were incubated with GST-IQGAP1 or GST alone immobilized on glutathione-Sepharose beads. WB: anti-MP1 antibody. Lysates not subjected to GST pull-down were processed in parallel (HeLa lysate). Black arrow indicates MP1 signal.
